Overexpression of Maize Glutathione S-Transferase ZmGST26 Decreases Drought Resistance of Arabidopsis

Drought stress critically endangers the growth and development of crops. Glutathione S-transferase plays a vital role in response to abiotic stress. However, there are few studies on glutathione maize drought In this study, significantly downregulated expression ZmGST26 roots under was analyzed by qRT-PCR. Promoter analyses showed that were several cis-acting elements related involved oxidative promoter region ZmGST26. Subcellular localization results localized nucleus. The transgenic lines Arabidopsis over-expressing more sensitive ABA seed germination inhibited ABA-mediated stomatal closure. Under stress, phenotypic rate, root length survival rate overexpressing lower than those wild-type lines. determination physiological biochemical indexes water loss malondialdehyde, O2− H2O2 overexpression increased compared with Arabidopsis, but antioxidant enzyme activities (CAT, SOD POD), proline chlorophyll contents reduced. Subsequently, qRT-PCR analysis stress-related gene that, conditions, levels DREB2A, RD29A, RD29B PP2CA genes Arabidopsis. summary, reduced resistance plants aggravating accumulation reactive oxygen species